| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, IHC, IHC-Fr, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | PE/Atto594 |
| Immunogen | Mouse IgA alpha heavy chain |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgA and Mouse Serum. No reaction was observed against other mouse or human heavy or light chain proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IGHA1 |
| Purity | Immunogen affinity purified |
| Application Notes | Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. |
| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
![Rabbit anti-Mouse IgA Secondary Antibody [PE/Atto594]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Rabbit anti-Mouse IgA Secondary Antibody [PE/Atto594]")
| Alkaline Phosphatase | NBP1-72684 | |
| Allophycocyanin/Cy7 | NBP2-60683APCCY7 | |
| Biotin | NBP1-73696 | |
| DyLight 350 | NBP2-60683UV | |
| DyLight 405 | NBP2-60683V | |
| DyLight 488 | NBP2-60683G | |
| DyLight 550 | NBP2-60683R | |
| DyLight 594 | NBP2-60683DL594 | |
| DyLight 650 | NBP2-60683C | |
| DyLight 680 | NBP2-60683FR | |
| DyLight 755 | NBP2-60683IR | |
| FITC | NBP1-73376 | |
| Janelia Fluor 549 | NBP2-60683JF549 | |
| Janelia Fluor 646 | NBP2-60683JF646 | |
| PE/Atto594 | NBP2-60683PEATT594 | |
| PE/Cy5.5 | NBP2-60683PECY55 | |
| PE/Cy7 | NBP2-60683PECY7 | |
| Rhodamine | NBP1-73374 | |
| Texas Red | NBP1-73702 |
Research Areas for IgA Antibody (NBP2-60683PEATT594)Find related products by research area.
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHA1 |
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Analysis in human cerebral cortex and tonsil tissues. Corresponding ATP6V0A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0015.jpg)
![Western Blot: ATP6V0A1 Antibody [NBP1-89342] - In Cln1-/- mice V0a1 is misrouted to plasma membrane preventing its interaction with AP-3. Pull-down assay with AP-3 antibody using total lysates from untreated (lane 1) and bromopalmitate-treated (lane 2) WT brain slices to detect V0a1 and its densitometric quantitation (n=4, *P<0.05). HEK-293 cells were transfected with WT GFP-V0a1 and GFP-V0a1-Cys25Ser mutant construct, and pull-down experiments were conducted with AP-3 antibody to detect GFP-V0a1,*P<0.05(n=4). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms14612), licensed under a CC-BY licence.](http://images.novusbio.com/images2/ATP6V0A1 Antibody-Western Blot-NBP1-89342-img0018.jpg)
![Western Blot: ATP6V0A1 Antibody [NBP1-89342] - In Cln1-/- mice V0a1 is misrouted to plasma membrane preventing its interaction with AP-3. Western blot analysis and densitometric quantitation of V0a1 in isolated plasma membrane fraction from WT and Cln1-/- mouse brain (n=4, *P<0.05). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms14612), licensed under a CC-BY licence.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Western-Blot-NBP1-89342-img0017.jpg)
![Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Western-Blot-NBP2-73857-img0001.jpg)
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human prostate tissue.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RFC2 antibody.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73857-img0002.jpg)
![Western Blot: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)---Azide-and-BSA-Free-Western-Blot-NBP2-73859-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.](http://images.novusbio.com/images2/RFC4 Antibody (OTI1A8) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-73859-img0006.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73859-img0005.jpg)
![Immunohistochemistry-Paraffin: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody RM119 at a 1:2000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA](http://images.novusbio.com/images2/IgG3-Antibody-(RM119)-[Unconjugated]-Immunohistochemistry-Paraffin-NBP2-62125-img0011.jpg)
![Immunohistochemistry: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - Immunohistochemistry of Human Lymphoid Tissue using NBP2-62125.](http://images.novusbio.com/images2/IgG3-Antibody-(RM119)-[Unconjugated]-Immunohistochemistry-NBP2-62125-img0003.jpg)
![ELISA: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - ELISA of human immunoglobulins shows NBP2-62125 only reacts to human IgG3. No cross reactivity with other human immunoglobulins, mouse, rat, or goat IgG.](http://images.novusbio.com/images2/IgG3-Antibody-(RM119)-[Unconjugated]-ELISA-NBP2-62125-img0005.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
