Goat anti-Monkey IgA Heavy Chain Secondary Antibody

Western Blot: Goat anti-Monkey IgA Secondary Antibody [Unconjugated] [NBP2-60680] - This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.

Western Blot: Goat anti-Monkey IgA Secondary Antibody [Unconjugated] [NBP2-60680] - Lane 1: Human IgA. Lane 2: None. Load: 100 ng per lane. Primary antibody: NBP2-60680 at 1:1000 and 4°C overnight. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Blocking Buffer: 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Monkey IgA. Other Band(s): None.

Western Blot: Goat anti-Monkey IgA Secondary Antibody [Unconjugated] [NBP2-60680] - Goat anti-Monkey IgA Secondary Antibody (Pre-absorbed) [NBP2-60680] - Lane 1: Human IgA. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 60 kDa for Monkey IgA. Other band(s): none. Image from the HRP version of this antibody.

Product Discontinued

Goat anti-Monkey IgA Heavy Chain Secondary Antibody Summary

• Immunogen: Goat anti-Monkey IgA Secondary Antibody was produced by repeated immunization with monkey IgA alpha heavy chain in goat.
• Specificity: No reaction was observed against Monkey IgG or Monkey IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other monkey heavy or light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This secondary antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgA and Monkey Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.