Rabbit anti-Mouse IgA Heavy Chain Secondary Antibody [Biotin] Summary
| Immunogen |
Mouse IgA alpha heavy chain |
| Specificity |
No reaction was observed against other Mouse heavy or light chain proteins. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Purity |
Multi-step |
Applications/Dilutions
| Dilutions |
- ELISA 1:20000 - 1:100000
- Immunohistochemistry 1:1000 - 1:5000
- Western Blot 1:2000 - 1:10000
|
| Application Notes |
This secondary antibody is suitable for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for other applications should be optimized by the end user. |
Packaging, Storage & Formulations
| Storage |
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative |
0.01% Sodium Azide |
| Concentration |
LYOPH |
| Purity |
Multi-step |
| Reconstitution Instructions |
Reconstitute with 1.0 ml deionized water (or equivalent). |
Notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgA and Mouse Serum
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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