Goat anti-Rat IgA Secondary Antibody [Texas Red]

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Western Blot: Goat anti-Mouse, Rat IgA Secondary Antibody [Texas Red] [NBP1-73680] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Texas Red
Concentration
LYOPH

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Catalog# & Conjugate Size Price

Goat anti-Rat IgA Secondary Antibody [Texas Red] Summary

Immunogen
Rat IgA heavy chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1% against other Rat heavy or light chain combinations.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 620, Excitation Wavelength: 596, Label Molecular Weight: 625, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Goat anti-Rat IgA Secondary Antibody [Texas Red]

  • IgA
  • Immunoglobulin A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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