Goat anti-Rat IgA Secondary Antibody [Biotin]

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Western Blot: Goat anti-Mouse, Rat IgA Secondary Antibody [Biotin] [NBP1-73678] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Goat anti-Rat IgA Secondary Antibody [Biotin] Summary

Immunogen
Rat IgA a chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against other Rat heavy or light chain proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
Application Notes
This product has been assayed against 1.0 ug of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:10,000 - 1:30,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Goat anti-Rat IgA Secondary Antibody [Biotin]

  • IgA
  • Immunoglobulin A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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