Goat anti-Human IgA Secondary Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Goat anti-Human IgA Secondary Antibody Summary

Immunogen
Human IgA
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with human IgA. This may cross react with IgA from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:30000
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Reviewed Applications
Read 1 Review rated 4
using
NB7441 in the following applications:

Publications
Read Publications using NB7441.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Human IgA Secondary Antibody

  • IgA
  • Immunoglobulin A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgA Antibody (NB7441)(2)

Review for IgA Antibody (NB7441) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7441:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence IgA NB7441
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reviewed by:
Natalie Erdmann
IF Human 09/06/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedMultiple myeloma cells lines LP-1 and MY5 cytospins
SpeciesHuman
LotA5

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/800 1ary antibody diluted in 10% donkey serum/PBS incubated O/N at 4oC.

Secondary Antibody

Secondary DescriptionDonkey F(ab")2 anti-goat IgG (H+L) Cy3
Secondary Manufacturer Cat#Jackson #705-166-147
Secondary Concentration1/2000

Details

Detection NotesZeiss AxioImager, Cy3 filter cube for 200 ms exposure
Fixation DetailsImmersion-fixed in 4% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step

Comments

CommentsHigh background, use at slightly higher dilution & wash well between steps. MY5 is positive control, LP-1 negative control.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgA Antibody (NB7441) (0)

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Additional Array Products

Diseases for IgA Antibody (NB7441)

Discover more about diseases related to IgA Antibody (NB7441).
 

Pathways for IgA Antibody (NB7441)

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Blogs on IgA.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

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1
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Natalie Erdmann
09/06/2016
Application: IF
Species: Human