Hyaluronan DuoSet ELISA, 5 Plate

• Reactivity: All-Multi
• Applications: ELISA
• Conjugate: Biotin

Hyaluronan DuoSet ELISA, 5 Plate Summary

• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hyaluronan DuoSet ELISA, 5 Plate

• Hyaluronan

Background

Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.

Publications for Hyaluronan (DY3614)(44)

Publications using DY3614

• R Chandrasek, C Mathieu, R Sheth, AP Cheng, D Fong, R McCormack, H El-Gabalaw, S Alishetty, M Paige, CD Hoemann UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism PLoS ONE, 2022;17(9):e0274420. 2022 [PMID: 36107941] (Human)
• AA Duizendstr, RJ De Knegt, NMA Nagtzaam, MGH Betjes, WA Dik, NHR Litjens, J Kwekkeboom Minimal Development of Liver Fibrosis in Adult Tolerant Liver Transplant Recipients Late After Immunosuppressive Drug Weaning and Transplantation Transplantation Proceedings, 2022;0(0):. 2022 [PMID: 36100485] (Human)
• S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department Scientific Reports, 2022;12(1):8733. 2022 [PMID: 35610344] (Human)
• RJ Krawetz, YE Wu, KL Bertram, A Shonak, AO Masson, G Ren, C Leonard, M Kapoor, JR Matyas, PT Salo Synovial mesenchymal progenitor derived aggrecan regulates cartilage homeostasis and endogenous repair capacity Cell Death & Disease, 2022;13(5):470. 2022 [PMID: 35585042] (Human)
• W Li, S Yang, P Xu, D Zhang, Y Tong, L Chen, B Jia, A Li, C Lian, D Ru, B Zhang, M Liu, C Chen, W Fu, S Yuan, C Gu, L Wang, W Li, Y Liang, Z Yang, X Ren, S Wang, X Zhang, Y Song, Y Xie, H Lu, J Xu, H Wang, W Yu SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network EBioMedicine, 2022;76(0):103861. 2022 [PMID: 35124429] (Human)
• AK Bongoni, IB Vikstrom, JL McRae, EJ Salvaris, N Fisicaro, MJ Pearse, S Wymann, T Rowe, AB Morelli, MP Hardy, PJ Cowan A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia-reperfusion injury Scientific Reports, 2021;11(1):21873. 2021 [PMID: 34750424] (Mouse)
• K Waeijen-Sm, NL Reynaert, RJHCG Beijers, S Houben-Wil, SO Simons, MA Spruit, FME Franssen Alterations in plasma hyaluronic acid in patients with clinically stable COPD versus (non)smoking controls Scientific Reports, 2021;11(1):15883. 2021 [PMID: 34354097] (Human)
• A Watkins, D Fasanello, D Stefanovsk, S Schurer, K Caracappa, A D'Agostino, E Costello, H Freer, A Rollins, C Read, J Su, M Colville, M Paszek, B Wagner, H Reesink Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models Bmc Veterinary Research, 2021;17(1):189. 2021 [PMID: 33980227] (Equine)
• GG Kellar, KA Barrow, LM Rich, JS Debley, TN Wight, SF Ziegler, SR Reeves Loss of versican and production of hyaluronan in lung epithelial cells are associated with airway inflammation during RSV infection J Biol Chem, 2020;0(0):. 2020 [PMID: 33187989] (Mouse)
• A Wolk, D Hatipoglu, A Cutler, M Ali, L Bell, J Hua Qi, R Singh, J Batoki, L Karle, VL Bonilha, O Wessely, H Stoehr, V Hascall, B Anand-Apte Role of FGF and Hyaluronan in Choroidal Neovascularization in Sorsby Fundus Dystrophy Cells, 2020;9(3):. 2020 [PMID: 32143276] (Human)
• E Galgoczi, F Jeney, M Katko, A Erdei, A Gazdag, L Sira, M Bodor, E Berta, B Ujhelyi, Z Steiber, F Gyory, EV Nagy Characteristics of Hyaluronan Synthesis Inhibition by 4-Methylumbelliferone in Orbital Fibroblasts Invest. Ophthalmol. Vis. Sci., 2020;61(2):27. 2020 [PMID: 32084270] (Human)
• CY Lin, C Kolliopoul, CH Huang, J Tenhunen, CH Heldin, YH Chen, P Heldin High levels of serum hyaluronan is an early predictor of dengue warning signs and perturbs vascular integrity EBioMedicine, 2019;0(0):. 2019 [PMID: 31526718] (Human)
• NA Lokman, ZK Price, EK Hawkins, AM Macpherson, MK Oehler, C Ricciardel 4-Methylumbelliferone Inhibits Cancer Stem Cell Activation and Overcomes Chemoresistance in Ovarian Cancer Cancers (Basel), 2019;11(8):. 2019 [PMID: 31443261] (Human)
• S Ishizuka, S Tsuchiya, Y Ohashi, K Terabe, EB Askew, N Ishizuka, CB Knudson, W Knudson Hyaluronan synthase 2 (HAS2) overexpression diminishes the procatabolic activity of chondrocytes by a mechanism independent of extracellular hyaluronan J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31270213] (Bovine)
• D Goody, SK Gupta, D Engelmann, A Spitschak, S Marquardt, S Mikkat, C Meier, C Hauser, JP Gundlach, JH Egberts, H Martin, T Schumacher, A Trauzold, O Wolkenhaue, S Logotheti, BM Pützer Drug Repositioning Inferred from E2F1-Coregulator Interactions Studies for the Prevention and Treatment of Metastatic Cancers Theranostics, 2019;9(5):1490-1509. 2019 [PMID: 30867845] (Human)
• Y Zhao, S Qiao, X Hou, H Tian, S Deng, K Ye, Y Nie, X Chen, H Yan, W Tian Bioengineered tumor microenvironments with naked mole rats high-molecular-weight hyaluronan induces apoptosis in breast cancer cells Oncogene, 2019;0(0):. 2019 [PMID: 30700830] (Human)
• R Gao, Y Liu, D Li, J Xun, W Zhou, P Wang, C Liu, X Li, W Shen, W Su, H Qiao, DG Stupack, N Luo PFKFB4 Promotes Breast Cancer Metastasis via Induction of Hyaluronan Production in a p38-Dependent Manner Cell. Physiol. Biochem., 2018;50(6):2108-2123. 2018 [PMID: 30415245] (Human)
• SR Reeves, KA Barrow, TK Kolstad, MP White, LM Rich, TN Wight, JS Debley Fibroblast gene expression following asthmatic bronchial epithelial cell conditioning correlates with epithelial donor lung function and exacerbation history Sci Rep, 2018;8(1):15768. 2018 [PMID: 30361541] (Human)
• M Marella, J Ouyang, J Zombeck, C Zhao, L Huang, RJ Connor, KB Phan, MC Jorge, MA Printz, RD Paladini, AB Gelb, Z Huang, GI Frost, BJ Sugarman, L Steinman, G Wei, HM Shepard, DC Maneval, PJ Lapinskas PH20 is not expressed in murine CNS and oligodendrocyte precursor cells Ann Clin Transl Neurol, 2017;4(3):191-211. 2017 [PMID: 28275653] (Mouse)
• Transcription factor TBX4 regulates myofibroblast accumulation and lung fibrosis J Clin Invest, 2016;0(0):. 2016 [PMID: 27400124] (Human)
• 4-methylumbelliferone diminishes catabolically-activated articular chondrocytes and cartilage explants via a mechanism independent of hyaluronan inhibition J Biol Chem, 2016;0(0):. 2016 [PMID: 27129266] (Human)
• Ishizuka S, Askew E, Ishizuka N, Knudson C, Knudson W 4-Methylumbelliferone Diminishes Catabolically Activated Articular Chondrocytes and Cartilage Explants via a Mechanism Independent of Hyaluronan Inhibition. J Biol Chem, 0;291(23):12087-104. 0 [PMID: 27129266] (Human)
• J Malaisse, V Pendaries, F Hontoir, V De Glas, D Van Vlaend, M Simon, C Lambert de, Y Poumay, B Flamion Hyaluronan Does Not Regulate Human Epidermal Keratinocyte Proliferation and Differentiation J. Biol. Chem, 2016;291(12):6347-58. 2016 [PMID: 26627828] (Human)
• Banerjee S, Modi S, McGinn O, Zhao X, Dudeja V, Ramakrishnan S, Saluja A Impaired Synthesis of Stromal Components in Response to Minnelide Improves Vascular Function, Drug Delivery, and Survival in Pancreatic Cancer. Clin Cancer Res, 2016;22(2):415-25. 2016 [PMID: 26405195] (Mouse)
• Kosinska M, Ludwig T, Liebisch G, Zhang R, Siebert H, Wilhelm J, Kaesser U, Dettmeyer R, Klein H, Ishaque B, Rickert M, Schmitz G, Schmidt T, Steinmeyer J Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species. PLoS ONE, 2015;10(5):e0125192. 2015 [PMID: 25933137] (Human)
• Jurado-Camino T, Cordoba R, Esteban-Burgos L, Hernandez-Jimenez E, Toledano V, Hernandez-Rivas J, Ruiz-Sainz E, Cobo T, Siliceo M, Perez de Diego R, Belda C, Cubillos-Zapata C, Lopez-Collazo E Chronic lymphocytic leukemia: a paradigm of innate immune cross-tolerance. J Immunol, 2015;194(2):719-27. 2015 [PMID: 25505275] (Human)
• Mueller A, Yoon B, Sadiq S Inhibition of hyaluronan synthesis protects against central nervous system (CNS) autoimmunity and increases CXCL12 expression in the inflamed CNS. J Biol Chem, 2014;289(33):22888-99. 2014 [PMID: 24973214] (Human)
• Huang Z, Zhao C, Chen Y, Cowell J, Wei G, Kultti A, Huang L, Thompson C, Rosengren S, Frost G, Shepard H Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation. J Immunol, 2014;192(11):5285-95. 2014 [PMID: 24778442] (Mouse)
• Malaisse J, Bourguignon V, De Vuyst E, Lambert de Rouvroit C, Nikkels A, Flamion B, Poumay Y Hyaluronan metabolism in human keratinocytes and atopic dermatitis skin is driven by a balance of hyaluronan synthases 1 and 3. J Invest Dermatol, 2014;134(8):2174-82. 2014 [PMID: 24658508] (Human)
• Yuan , Han, Tank , Mihir, Alsofyani , Abeer, Shah , Naman, Talati , Nishant, LoBello , Jaclyn C, Kim , Jin Ryou, Oonuki , Yoji, de la Motte , Carol A, Cowman , Mary K Molecular mass dependence of hyaluronan detection by sandwich ELISA-like assay and membrane blotting using biotinylated hyaluronan binding protein. Glycobiology, 2013;23(11):1270-80. 2013 [PMID: 23964097] (N/A)
• Chowdhury , Biswajit, Hemming , Richard, Hombach-Klonisch , Sabine, Flamion , Bruno, Triggs-Raine , Barbara Murine hyaluronidase 2 deficiency results in extracellular hyaluronan accumulation and severe cardiopulmonary dysfunction. J Biol Chem, 2013;288(1):520-8. 2013 [PMID: 23172227] (Mouse)
• Jiang P, Li X, Thompson CB Effective targeting of the tumor microenvironment for cancer therapy. Anticancer Res., 2012;32(4):1203-12. 2012 [PMID: 22493350] (Human)
• Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients. Mucosal Immunol, 0;5(2):140-9. 0 [PMID: 22189956] (Human)
• Guo N, Woeller CF, Feldon SE, Phipps RP Peroxisome Proliferator-activated Receptor gamma Ligands Inhibit Transforming Growth Factor-beta-induced, Hyaluronan-dependent, T Cell Adhesion to Orbital Fibroblasts. J. Biol. Chem., 2011;286(21):18856-67. 2011 [PMID: 21454487] (Human)
• Schledzewski K, Geraud C, Arnold B, Wang S, Grone HJ, Kempf T, Wollert KC, Straub BK, Schirmacher P, Demory A, Schonhaber H, Gratchev A, Dietz L, Thierse HJ, Kzhyshkowska J, Goerdt S Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors. J. Clin. Invest., 2011;121(2):703-14. 2011 [PMID: 21293057] (Mouse)
• van der Windt GJ, Florquin S, de Vos AF CD44 deficiency is associated with increased bacterial clearance but enhanced lung inflammation during Gram-negative pneumonia. Am. J. Pathol., 2010;177(5):2483-94. 2010 [PMID: 20864681] (Mouse)
• Guo N, Li X, Mann MM, Funderburgh ML, Du Y, Funderburgh JL Hyaluronan synthesis mediates the fibrotic response of keratocytes to transforming growth factor beta. J. Biol. Chem., 2010;285(42):32012-9. 2010 [PMID: 20685654] (Bovine)
• Craig EA, Craig E, Austin A, Austin AF, Vaillancourt RR, Vaillancourt R, Barnett JV, Camenisch T, Camenisch TD TGFbeta2-mediated production of hyaluronan is important for the induction of epicardial cell differentiation and invasion. Exp Cell Res, 0;316(20):3397-405. 0 [PMID: 20633555] (Mouse)
• Guo N, Baglole CJ, O'Loughlin CW, Feldon SE, Phipps RP Mast cell-derived prostaglandin D2 controls hyaluronan synthesis in human orbital fibroblasts via DP1 activation: implications for thyroid eye disease. J. Biol. Chem., 2010;285(21):15794-804. 2010 [PMID: 20308056] (Human)
• Motz GT, Eppert BL, Wesselkamper SC, Flury JL, Borchers MT Chronic cigarette smoke exposure generates pathogenic T cells capable of driving COPD-like disease in Rag2-/- mice. Am. J. Respir. Crit. Care Med., 2010;181(11):1223-33. 2010 [PMID: 20133926] (Mouse)
• Babelova A, Moreth K, Tsalastra-Greul W, Zeng-Brouwers J, Eickelberg O, Young MF, Bruckner P, Pfeilschifter J, Schaefer RM, Schaefer R, Grone HJ, Schaefer L Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors. J Biol Chem, 0;284(36):24035-48. 0 [PMID: 19605353] (Mouse)
• de la Motte C, Nigro J, Vasanji A, Rho H, Kessler S, Bandyopadhyay S, Danese S, Fiocchi C, Stern R Platelet-derived hyaluronidase 2 cleaves hyaluronan into fragments that trigger monocyte-mediated production of proinflammatory cytokines. Am. J. Pathol., 2009;174(6):2254-64. 2009 [PMID: 19443707] (Human)
• van Steensel L, Paridaens D, Schrijver B, Dingjan GM, van Daele PL, van Hagen PM, van den Bosch WA, Drexhage HA, Hooijkaas H, Dik WA Imatinib mesylate and AMN107 inhibit PDGF-signaling in orbital fibroblasts: a potential treatment for Graves' ophthalmopathy. Invest. Ophthalmol. Vis. Sci., 2009;50(7):3091-8. 2009 [PMID: 19234339] (Human)
• Aytekin M, Comhair SA, de la Motte C, Bandyopadhyay SK, Farver CF, Hascall VC, Erzurum SC, Dweik RA High levels of hyaluronan in idiopathic pulmonary arterial hypertension. Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(5):L789-99. 2008 [PMID: 18776053] (Human)

FAQs for Hyaluronan (DY3614). (Showing 1 - 1 of 1 FAQs).

1. Can you tell me if the Hyaluronan DouSet ELISA will is suitable to use with pig plasma?
   • Yes, this ELISA is for any species.