Human Respiratory Syncytial Virus (RSV) phosphoprotein Antibody (3-5-18)

Western Blot: Human Respiratory Syncytial Virus (RSV) phosphoprotein Antibody (RSV 3-5-18) [NBP2-50244] - The antigen used was gradient-purified RSN-2 virus in lanes 1 and 2. First antibodies: Lane 1: anti-RSV Phosphoprotein VP32 [3-5-18] antibody; Lane 2: RS virus convalescent human sera. Indirect immunofluorescence: staining of RSN-2 virus infected unfixed and fixed BSC-1 cells (Gimenez et al., 1984).

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clone: 3-5-18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Human Respiratory Syncytial Virus (RSV) phosphoprotein Antibody (3-5-18) Summary

• Immunogen: The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984).
• Specificity: Not reactive with other member of genus Pneumovirus (Pneumonia virus of mice). Antibody reactive with VPP32 from human RSV of subgroup A and bovine respiratory syncytial virus (BRS)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Notes

Positive control(s): Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Lane 1: 3-5 antibody. Lane2: RSV convalescent human sera.Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.