Human Regulatory T Cell Flow Cytometry Kit


Flow Cytometry: Human Regulatory T Cell Flow Cytometry Kit [FMC021-NOV] - Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A Mouse Anti-Human CD4 more

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Human Regulatory T Cell Flow Cytometry Kit Summary

This product is designed for the flow cytometric analysis of human regulatory T cells using three fluorochrome-conjugated antibodies.

Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Store at 4C. Do not freeze.

Kit Components

  1. Positive Marker: Human CD4-FITC Mouse IgG2A; Clone 11830
  2. Positive Marker: Human IL-2 R alpha/CD25-APC Mouse IgG2A; Clone 24212
  3. Positive Marker: Human/Mouse FoxP3-PE Rabbit IgG; Clone 1054C
  4. Isotype Control: Rabbit IgG Control-PE
  5. FoxP3/Transcription Factor Permeabilization & Wash Buffer (10X)
  6. FoxP3/Transcription Factor Fixation Concentrate (4X)
  7. FoxP3/Transcription Factor Fixation Diluent
  8. Staining Buffer (1X)


The primary function of regulatory T cells, also known as suppressor T cells, is to maintain immune homeostasis. This involves suppression of successful immune responses and control of self versus non-self recognition. Failure of the latter results in autoimmune destruction of host cells and tissue. Like other T cells, regulatory T cells mature in the thymus where they are characterized by the variable expression of CD8, CD4, CD25 and FoxP3. The importance of FoxP3 is underlined by genetic mutations in this molecule which result in a fatal autoimmune disorder known as Immune dysregulation, Polyendocrinopathy, Enteropathy X-linked (IPEX) syndrome. Conversely, taking advantage of the immunosuppressive potential of regulatory T cells is an important area for advancement in the fields of autoimmune disease and organ transplantation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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