Human Naive CD4+/CD45RO- T Cell Column Enrichment Kit

Product Discontinued

Human Naive CD4+/CD45RO- T Cell Column Enrichment Kit Summary

• Description: Designed to isolate sub-populations of CD4+ /CD45RO- human T cells via high affinity negative selection. The resulting column eluate is a highly enriched T cell subset population with minimal contamination of either CD45RO+ or CD8+ cells.
  
  Features
  • Yields a highly pure (93-96%) population of CD4+/CD45RO- naive T cells
  • Separates cells based on gravitational flow
  • Negative selection reduces sources of experimental variation
• Kit Type: Enrichment Kit

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Human CD4+ T cells develop in the thymus and are released as naive T cells that express low levels of CD45RO. These cells differentiate into subsets of specialized effector T lymphocytes in response to distinct environmental cues and the activation of specific transcription factors. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, Th17, Th9, Th22, and regulatory T cells. CD4+ T cell subsets secrete characteristic combinations of cytokines which enables them to exert diverse functions including the recruitment and activation of additional immune cells, the dampening of ongoing immune responses, and the maintenance of immunologic memory.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.