Human Mesenchymal Stem Cell Flow Cytometry Kit

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Flow Cytometry: Human Mesenchymal Stem Cell Flow Cytometry Kit [FMC020-NOV] - KG-1 acute myelogenous leukemia cells (A) and peripheral blood mononuclear cells (PBMCs) (B) were stained using the Negative Marker Cocktail ...read more
Flow Cytometry: Human Mesenchymal Stem Cell Flow Cytometry Kit [FMC020-NOV] - Human bone marrow-derived mesenchymal stem cells were stained using the antibodies and reagents provided in the Human Mesenchymal Stem Cell ...read more
N/A: Human Mesenchymal Stem Cell Flow Cytometry Kit [FMC020-NOV] - Human mesenchymal stem cells were cultured in StemXVivo (TM) Mesenchymal Stem Cell Expansion Media and differentiation was induced as indicated using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
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Human Mesenchymal Stem Cell Flow Cytometry Kit Summary

Description
This product is designed for the flow cytometric analysis of human MSCs using three fluorochrome-conjugated antibodies and one fluorochrome-conjugated antibody cocktail.

Features
  • Includes all of the antibodies needed to fulfill the ISCT's minimal criteria for the definition of human MSCs
  • Can be used with the Human MSC Functional ID kit to define MSCs according to the ISCT recommendations
  • Provides a cost-effective method to analyze several MSC markers in a single sample
  • Reveals heterogeneity in the starting cell population

Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.

Kit Components

Components
  1. 125 mL of 1X Staining Buffer
  2. Negative Marker Cocktail: 500 uL of Negative Marker Cocktail (CD45-PE Mouse IgG1; Clone 2D1, CD34-PE Mouse IgG1; Clone QBEnd10, CD11b-PE Mouse IgG2B; Clone 238446, CD79A-PE Mouse IgG1; Clone 706931, HLA-DR-PE Mouse IgG1; Clone L203)
  3. Negative Isotype Control Cocktail: 500 uL of Negative Isotype Cocktail (Mouse IgG1-PE Isotype Control; Clone 11711, Mouse IgG2B-PE Isotype Control; Clone 133303)
  4. Positive Marker: 500 uL of CD105-PerCP Mouse IgG1; Clone 166707
  5. Positive Marker: 500 uL of CD73-CFS Mouse IgG2B; Clone 606112
  6. Positive Marker: 500 uL of CD90-APC Mouse IgG2A; Clone Thy-1A1
  7. Positive Isotype Control: 500 uL of Mouse IgG1-PerCP; Clone 11711
  8. Positive Isotype Control: 500 uL of Mouse IgG2A-APC; Clone 20102
  9. Positive Isotype Control: 500 uL of Mouse IgG2B-CFS; Clone 133303

Background

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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