Human Mesenchymal Stem Cell Flow Cytometry Kit

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Flow Cytometry: Human Mesenchymal Stem Cell Flow Cytometry Kit [FMC002-NOV] - Undifferentiated (A) and osteocyte-differentiated (B) human mesenchymal stem cells were stained with the indicated antibodies (filled ...read more

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Summary
Reactivity HuSpecies Glossary
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Human Mesenchymal Stem Cell Flow Cytometry Kit Summary

Description
This product is designed for the flow cytometric analysis of human MSCs using four fluorochrome-conjugated antibodies.

Features
  • Verifies MSC identity in less than 2 hours
  • Conjugated antibodies allow for single step staining
  • Defines the starting cell population
  • Multiple markers reduce experimental variation

Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.

Kit Components

Components
  1. 250 uL of Mouse IgG1-PerCP Isotype Control
  2. 250 uL of Mouse IgG2A-APC Isotype Control
  3. 250 uL of Mouse IgG1-CFS Isotype Control
  4. 250 uL of Mouse IgG1-PE Isotype Control
  5. 100 mL of 1X Staining Buffer
  6. Positive Marker: 250 uL of CD105/Endoglin-PerCP Mouse IgG1; Clone 166707
  7. Positive Marker: 250 uL of CD146/MCAM-CFS Mouse IgG1; Clone 128018
  8. Positive Marker: 250 uL of CD90/Thy1-APC Mouse IgG2A; Clone Thy-1A1
  9. Negative Marker: 250 uL of CD45-PE Mouse IgG1; Clone 2D1

Background

The term "mesenchymal stem cells" (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term "multipotent mesenchymal stromal cells" is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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