Human IgG, Fab2 Native Protein

SDS-Page: Human IgG, Fab2 Full length Protein [NBP1-96779] - Lane 1: Human F(ab')2 - Non-Reduced. Lane 2: Human F(ab')2 - Reduced. Load: 1.0 ug per lane.

SDS-Page of Human IgG F(ab')2 Fragment. Lane 1: Human F(ab')2 - Non-Reduced. Lane 2: Human F(ab')2 - Reduced. Load: 1.0 ug per lane. Predicted/observed size: 25 kDa - Reduced, 120 kDa - Non-Reduced for F(ab')2 fragment. Other band(s): None.

• Reactivity: Hu
• Applications: WB, ELISA, PAGE

Human IgG, Fab2 Native Protein Summary

• Description: Native Human Human IgG, Fab2
  
  Source: Normal serum (Accession #:CAI39859.1)
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
  
  No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(ab')2. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:50000
  • SDS-Page 1:100-1:2000
  • Western Blot 1:1000
• Application Notes: This product has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Background

Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Human IgG F(ab')2 molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.