Human Hematopoietic Progenitor Cell Flow Cytometry Kit

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Flow Cytometry: Human Hematopoietic Progenitor Cell Flow Cytometry Kit [FMC019-NOV] - Human umbilical cord blood cells were stained using reagents supplied in this kit. Cells were simultaneously analyzed for the ...read more
Flow Cytometry: Human Hematopoietic Progenitor Cell Flow Cytometry Kit [FMC019-NOV] - Human umbilical cord blood cells were stained with the indicated antibodies included in this kit. Representative expression of each ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
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Human Hematopoietic Progenitor Cell Flow Cytometry Kit Summary

Description
This product is designed for single-step, flow cytometric analysis of human HPCs using four fluorochrome-conjugated antibodies.

Features
  • Verifies human hematopoietic progenitor cell identity within 2 hours
  • Cost-effective kit provides an optimized combination of antibodies
  • Multiple cell-surface markers reduce experimental variability
  • Defines a starting cell population

Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. Positive Marker: 250 uL of CD34-APC Mouse IgG1; Clone QBEnd10
  2. Positive Marker: 250 uL of SCF R/CD117-PE Mouse IgG1; Clone 47233
  3. Positive Isotype Control: 250 uL of Mouse IgG1-APC ; Clone 11711
  4. Positive Isotype Control: 250 uL of Mouse IgG1-PE; Clone 11711
  5. Negative Marker: 250 uL of CD11b-CFS Mouse IgG2B; Clone 238446
  6. Negative Marker: 250 uL of CD38-PerCP Mouse IgG2A; Clone 240742
  7. Negative Isotype Control: 250 uL of Mouse IgG2A-PerCP; Clone 20102
  8. Negative Isotype Control: 250 uL of Mouse IgG2B-CFS; Clone 133303
  9. 100 mL of 1X Staining Buffer

Background

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long maintenance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continuously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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