Human CD4+ T Cell Isolation Kit

Flow Cytometry: Human CD4+ T Cell Isolation Kit [MAGH102-NOV] - Enrichment of CD4+ T Cells from Human Peripheral Blood Mononuclear Cells Using the MagCellect CD4+ T Cell Isolation Kit. Ficolled human PBMC before (A) and after (B) isolation of CD4+ T cells were stained with PE-conjugated Mouse Anti-Human CD3 epsilon Monoclonal Antibody and FITC-conjugated Mouse Anti-Human CD4 Monoclonal Antibody.

Flow Cytometry: Human CD4+ T Cell Isolation Kit [MAGH102-NOV] - Ficolled human PBMCs before (A) and after (B) isolation of CD4+ T cells using the MagCellect CD4+T Cell Isolation Kit. Dot plots reflect double-staining of all viable cells with CD4-FITC/CD3-PE.

Product Discontinued

Human CD4+ T Cell Isolation Kit Summary

• Description: The MagCellect Human CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD4+ T cells. Typical recovery ranges from 45-65% and the purity of recovered CD4+ T cells ranges from 90-97%, with no detectable CD8+ T cells.
  
  Features
  • Yields highly pure (90-97%) CD4+ T cells in under an hour
  • Isolated CD4+ T cells are untouched by beads or antibodies
  • Cost effective for the processing of multiple samples in combination with Magnet (Catalog # MAG997-NOV)
  • Negative selection reduces sources of experimental variation
• Kit Type: Isolation Kit
• Gene: CD4

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th9, Th17,Th22, Tfh, and Treg cells which regulate humoral and cellular immunity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1. MagCellect products are designed for the isolation of cells in a "liquid phase". MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD4