Human CD4+ T Cell Column Enrichment Kit

Product Discontinued

Human CD4+ T Cell Column Enrichment Kit Summary

• Description: Designed to prepare sub-populations of CD4 human T cells via high affinity negative selection. The resulting column eluate is a highly enriched CD4+ cell subset population with minimal depletion of the selected T cells
  
  Features
  • Yields a highly pure (88-95%) population of CD4+ T cells
  • Separates cells based on gravitational flow
  • Negative selection reduces sources of experimental variation
• Kit Type: Enrichment Kit
• Gene: CD4

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th9, Th17,Th22, Tfh, and Treg cells which regulate humoral and cellular immunity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD4