Human CD4+ CD25+ Regulatory+ T Cell Isolation Kit

Flow Cytometry: Human CD4+ CD25+ Regulatory+ T Cell Isolation Kit [MAGH104-NOV] - Enrichment of CD4+ T cells (Step 1) and CD4+CD25+ Cells (Step 2) were stained using the conjugated antibodies provided in this kit. In Step 1, CD4+ T cells were isolated from PBMC. The additional reagent, Anti-Human CD3 epsilon-PE Antibody was used in this step. In Step 2, CD4+CD25+ T cells were isolated from the CD4+ cells recovered in Step 1.

Product Discontinued

Human CD4+ CD25+ Regulatory+ T Cell Isolation Kit Summary

• Description: The MagCellect Human CD4+CD25+ Regulatory T Cell Isolation Kit is designed to isolate human CD4+CD25+ regulatory T cells from a preparation of peripheral blood mononuclear cells (PBMC) using a two-step procedure that combines both negative and positive selection techniques. The resulting cell preparation is highly enriched for CD4+CD25+ T cells with a purity of recovered cells ranging between 85-95%.
  
  Features
  • Yields highly enriched populations (85-95%) of Treg cells
  • Does not require specialized instrumentation
  • Obtains isolated Treg cells by a combination of negative and positive selection
• Kit Type: Isolation Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

The primary function of regulatory T cells, also known as suppressor T cells, is to maintain immune homeostasis. This involves suppression of successful immune responses and control of self versus non-self recognition. Failure of the latter results in autoimmune destruction of host cells and tissue. Like other T cells, regulatory T cells mature in the thymus where they are characterized by the variable expression of CD8, CD4, CD25 and FoxP3. The importance of FoxP3 is underlined by genetic mutations in this molecule which result in a fatal autoimmune disorder known as Immune dysregulation, Polyendocrinopathy, Enteropathy X-linked (IPEX) syndrome. Conversely, taking advantage of the immunosuppressive potential of regulatory T cells is an important area for advancement in the fields of autoimmune disease and organ transplantation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.