Human Blood B Booster Kit

B Cell Stimulation: Human Blood B Booster Kit [DDXK-HuBBB] - Microscopic observation during the time course of the culture.

• Reactivity: Hu
• Applications: Func

Human Blood B Booster Kit Summary

• Description: Dendritics has developed the HuBBB(R) kit as an improvement of the above approaches, in order to target the largest B cell population from peripheral blood, thus offering the opportunity to pursue studies on immortalized B cells representative of the overall peripheral B repertoire. Under the best conditions, the kit allows the immortalization of up to 20% of the starting B cell population. The HuBBB(R) kit was packaged so as to make it very easy to handle for the scientists ; it contains all the reagents needed to obtain from 5.104 to 2.105 independent B cell clones from 107 peripheral blood mononuclear cells using ten 96 wells culture plates. The resulting cells are available for a wide range of studies including genomic, transcriptomic and proteomic fields.
  
  Intended usage: -Production of human monoclonal antibodies for research use. -Drug discovery/development: development of fully human antibodies for use in in vitro studies. -Evaluation of biotherapeutics agents or candidate vacines on immunoglobulin secretion. -Measurment of auto/allo-antibodies against specific biological targets like cytokines. Successful Proofs of Concepts: -Fully human monoclonal antibodies were made against several targets including HuMAB anti-HPA5b, -IL1a, -BetV1, -Derp1 -Neutralizing HumAB anti-Chik -Hu and canMab anti-Leptospira
• Kit Type: Kit

Applications/Dilutions

• Dilutions:
  • B Cell Stimulation
• Application Notes: Intended use: Immortalization of human B lymphocytes from peripheral blood samples

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Human Blood B Booster Kit

• Blood B Booster kit
• Blood B Booster
• human B cell immortalization Human Blood B Booster
• Human B cell immortalization kit
• Human Blood B Booster kit
• Human Blood B Booster

Background

Several approaches have been developed by scientists aiming to study the physiology of circulating B cells. They are generally limited both by the low number of B cells (8-10% of peripheral blood mononuclear cells) and by their low capacity to enter cell cycle. The most successful approaches of these last decades were the CD40 system (1), a culture technique allowing the long-term growth of B lymphocytes, and the B cell transformation using the Epstein-Barr virus (EBV) (2;3;4). However, the efficiency of these methods remains limited, targeting only 1% (CD40) and 1/106 (EBV) of the B lymphocytes. An improved method has recently been reported but targeting only a very restricted IgG+CD22+ B cell population (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Human Blood B Booster Kit (DDXK-HuBBB)(1)

Publication using DDXK-HuBBB

• Ndongo-Thiam N et al. Negative association between autoantibodies against IL-17, IL-17/anti-IL-17 antibody immune complexes and destruction in rheumatoid arthritis. Ann Rheum Dis 2016-07-01 [PMID: 27130907]