Human B Cell Isolation Kit

Images

 
Flow Cytometry: Human B Cell Isolation Kit [MAGH103-NOV] - Isolation of human CD19+ B cells from PBMC and whole blood using MagCellect Human B Cell Isolation kit. Human PBMC (top) and whole blood (bottom) were prepared ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Kit Type
Isolation Kit

Order Details

Human B Cell Isolation Kit Summary

Description
The MagCellect Human B Cell Isolation Kit is designed to isolate B cells via a negative selection principle. The resulting cell preparation is highly enriched with B cells. Typical recovery of the targeted cell population ranges from 45-65% and the purity of recovered B cells ranges from 90-98%.

Kit Type
Isolation Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.0 ml of a Human B Cell Biotinylated Antibody Cocktail
  2. 1.25 ml of a Streptavidin Ferrofluid
  3. 10 ml of a 10X Buffer

Background

B cells are a subset of lymphocytes that express immunoglobulins on their cell surface. The B cell population is a vital component of the humoral immune response. B cell activation leads to antigen uptake and presentation, affinity maturation and class switching of the immunoglobulins, and antibody secretion. B cells represent a small portion (~5%) of the peripheral lymphocyte population making it difficult to obtain sufficient B cell quantities for many research applications. The ability to isolate and expand B cell populations ex vivo is valuable to researchers who require B cells for downstream applications including high throughput B cell assays and ex vivo differentiation of T cells. MagCellect products are designed for the isolation of cells in a "liquid phase". MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Human B Cell Kit (MAGH103-NOV) (0)

There are no publications for Human B Cell Kit (MAGH103-NOV).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Human B Cell Kit (MAGH103-NOV) (0)

There are no reviews for Human B Cell Kit (MAGH103-NOV). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human B Cell Kit (MAGH103-NOV) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Human B Cell Products

Array MAGH103-NOV

Blogs on Human B Cell

There are no specific blogs for Human B Cell, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Human B Cell Isolation Kit and receive a gift card or discount.