| Details of Functionality | The Human 26S Proteasome can be used for in vitro degradation of appropriate substrates. The Human 26S Proteasome should be used immediately after thawing since it is inherently labile and will dissociate into free 20S and 19S subcomplexes over time. Reaction conditions will need to be optimized for each specific application. We recommend an initial Human 26S Proteasome concentration of 2-20 nM. |
| Source | Human embryonic kidney cell, HEK293-derived 26S Proteasome protein |
| Protein/Peptide Type | Natural Enzymes |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
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| Theoretical MW | 2,100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | X mg/ml (X μM) in 20 mM HEPES pH 7.5, 20 mM NaCl, 2 mM Mg-ATP, 15% (v/v) Glycerol |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
The 26S Proteasome is the major non-lysosomal protease in eukaryotic cells and is responsible for the degradation of ubiquitinated substrates and misfolded proteins. It is composed of two subcomplexes: the 20S Proteasome core particle and the 19S Proteasome regulatory particle. The 20S Proteasome facilitates proteolytic cleavage of protein substrates and is composed of 28 subunits arranged into four stacked rings (1,2). The outer rings of the 20S Proteasome are composed of seven related but non-identical, non-catalytic subunits, alpha1-7, that form a gate and restrict substrate access. The inner rings of the 20S Proteasome are composed of seven related but non-identical subunits, beta1-7. Beta1, 2, and 5 have proteyolytic activity. The 19S Proteasome caps one or both ends of the core particle and regulates substrate access to the catalytic core in an ATP-dependent manner by modulating 20S Proteasome conformation (3-5). The 19S Proteasome consists of a base subcomplex and a lid subcomplex. The base subcomplex is composed of six AAA+ family members, scaffolding proteins, and regulatory proteins involved in Ubiquitin recognition (2,6). The 19S Proteasome lid subcomplex contains eight subunits plus one deubiquitinating enzyme, Rpn11 (2,6). Small molecules that inhibit the activity of the 26S Proteasome are used to study the function of short-lived intracellular proteins and for the clinical treatment of certain forms of cancer (7). This highly purified 26S Proteasome preparation (from the transformed HEK cell line) can be used in vitro for the degradation of peptide substrates and poly-ubiquitinated proteins.
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Using Ubiquitin Antibodies in Various Disease Research Ubiquitin is a small, highly conserved protein which plays an important role in protein breakdown, covalently bonding to proteins to mark them for proteolytic degradation in a process called ubiquitination. Ubiquitin also binds to inclusion bodies (ac... Read full blog post. |
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