HT-29 Whole Cell Lysate

Product Discontinued

HT-29 Whole Cell Lysate Summary

• Description: GROWTH MEDIA: ATCC medium (McCoy's 5a medium with 1.5 mM L-glutamine, and 10% FBS)
• Specificity: HT-29 (Human Colorectal Adenocarcinoma)

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
• Buffer: SDS sample buffer containing 50 mM DTT.
• Concentration: 2 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Digestive/Waste
• Tissue Condition: Adenocarcinoma
• Subcellular Fraction: Whole

Background

HT-29 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HT-29 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for HT-29 Lysate (NBP2-03963)(1)

Publication using NBP2-03963

• Gerlach JC, Foka HG, Thompson RL et al. Epithelial Cell Adhesion Molecule Fragments and Signaling in Primary Human Liver Cells J. Cell. Physiol. 2017-11-18 [PMID: 29150960] (WB, Human)