![Western Blot: HSV Tag Antibody [HRP] [NB600-515] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/HSV-Tag-Antibody-[HRP]-Western-Blot-NB600-515-img0001.jpg "Western Blot: HSV Tag Antibody [HRP] [NB600-515] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
| Immunogen | Goats were immunized with HSV (SQPELAPEDPED) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. ELISA - 1:10,000-1:100,000 <br>Western Blots Colorimetric detection: 1:1,000 - 1:10,000 Chemiluminescent detection: 1:1,000 - 1:30,000 <br>Immunocytochemistry: 1:200 - 1:500 In some cases, the antibody may be diluted further than indicated. |
| Readout System |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS and 0.2% BSA |
| Preservative | 0.05% Pro-Clean 400 |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HSV Tag Antibody (NB600-515)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
