Human Hsp60 Autoantibody (IgG, IgA, IgM) ELISA Kit (Colorimetric)

ELISA: Human Hsp60 Autoantibody (IgG, IgA, IgM) ELISA Kit (Colorimetric) [NBP2-62137] - A typical standard curve for Hsp60 Autoantibody (IgG, IgA, IgM) ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Human serum, human plasma
• Standard Curve Range: 7.81 - 250 ng/ml
• Sensitivity: 2.88 ng/ml

Human Hsp60 Autoantibody (IgG, IgA, IgM) ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 7.81 - 250 ng/ml
• Sensitivity: 2.88 ng/ml
• Assay Type: Direct ELISA
• Inter-Assay: The Inter-Assay coefficient of variation of the Anti-Human Hsp60 (total) ELISA was determined to be <10%.
• Intra-Assay: The Intra-Assay coefficient of variation of the Anti-Human Hsp60 (total) ELISA was determined to be <10%.
• Spike Recovery: N/A
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Hsp60 Autoantibody (IgG, IgA, IgM) ELISA Kit (NBP2-62137)(1)

Publication using NBP2-62137

• Iacomino N, Scandiffio L, Conforti F et al. Muscle and Muscle-like Autoantigen Expression in Myasthenia Gravis Thymus: Possible Molecular Hint for Autosensitization Biomedicines 2023-02-28 [PMID: 36979710] (ELISA)

FAQs for Hsp60 Autoantibody (IgG, IgA, IgM) ELISA Kit (NBP2-62137). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.