Human Hsp27/F13A1 ELISA Kit (Colorimetric)

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ELISA: Human Hsp27/F13A1 ELISA Kit (Colorimetric) [NBP2-82498] - Samples were spiked with high concentrations of Human Hsp27/F13A1 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human Hsp27/F13A1 ELISA Kit (Colorimetric) [NBP2-82498] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
18.75 - 1200 pg/mL
Sensitivity
11.25 pg/mL

Order Details

Human Hsp27/F13A1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Hsp27/F13A1 in samples. No Significant cross-reactivity or interference between Human Hsp27/F13A1 and analogues was observed.
Standard Curve Range
18.75 - 1200 pg/mL
Sensitivity
11.25 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.02
Intra-Assay
CV% < 4.91
Spike Recovery
87-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Hsp27/F13A1 ELISA Kit (Colorimetric)

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • Hsp25
  • HSP27HSP 27
  • HSP28CMT2F
  • HspB1
  • SRP27
  • Stress-responsive protein 27

Background

The protein encoded by the Hsp27 / F13A1 gene is induced by environmental stress and developmental changes. The encoded protein isinvolved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stressinduction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motorneuropathy (dHMN). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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