| Description | The HRE/LUCPoter(TM) reporter cell line is designed to monitor the induction of HRE and can be used for screening of agonists, antagonists or signaling inhibitors of HRE induction (Hypoxia) as well as for studying the hypoxia-related signaling pathways.
Contents: 3-4 x 10^6 cells Biosafety Level: 2 |
| Immunogen | The HRE/LUCPorter(TM) reporter cell line is a stably transfected HeLa cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the hypoxia response element (HRE). In response to hypoxia (low oxygen), HREs of target genes are recognized and regulated by the hypoxia-inducible factors (HIFs) which belong to the family of basic helix-loop-helix transcription factors and form heterodimeric complex comprising the alpha subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3), among which HIF-1 alpha and HIF-2 alpha are predominant isoforms. Activation of HIFs can also be mediated by chemical hydroxylase inhibitors as hypoxia mimetics including the iron chelator desferrioxamine and cobalt chloride. As also shown in Figure 1, the HRE activity in the HRE/LUCPorter(TM) reporter cell line was induced by cobalt chloride in a dose response manner (Figure 1). |
| Selection Agent | Puromycin at 1 ug/ml |
| Growth Properties | Adherent Morphology : Epithelial |
| RCL Type | Luciferase - (LUCPorter™) |
| Host | HeLa |
| Storage | Store in gas phase of liquid nitrogen. |
| Reconstitution Instructions | Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin (Note: The selection agents for this cell line is puromycin at 1 ug/ml). |
