Recombinant Parasite HpARI His Protein

ELISA: Recombinant HpARI His Protein [NBP3-18165] - Binding of HpARI (Alarmin Release Inhibitor) (rec.) (His) to human and mouse IL-33.Methods: IL-33 (human) (rec.) (untagged) (rec.) (untagged) as well as an unrelated control protein are coated on an ELISA plate at 1ug/ml. After blocking and washing steps, indicated concentrations of HpARI (Alarmin Release Inhibitor) (rec.) (His) are added. Following incubation for 1 h at RT, the binding was detected using an anti-His antibody (HRP).

• Reactivity: Pa
• Applications: ELISA, Bioactivity
• Concentration: LYOPH

Recombinant Parasite HpARI His Protein Summary

• Description: Heligmosomoides polygyrus protein HpARI (aa 17-251) fused at the C-terminus to a His-tag..
  
  Source: HEK 293 cells
• Specificity: Binds to human and mouse IL-33.
• Details of Functionality: Binds to human and mouse IL-33
• Source: HEK293
• Protein/Peptide Type: Recombinant Protein
• Purity: >90% SDS-PAGE
• Endotoxin Note: <0.01EU/ug purified protein (LAL test; Lonza).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • ELISA
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Heligmosomoides polygyrus. Cross-reactive with human, mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >90% SDS-PAGE
• Reconstitution Instructions: Reconstitute with 50 ul sterile water (After reconstitution: 1mg/ml)

Alternate Names for Recombinant Parasite HpARI His Protein

• H. polygyrus Alarmin Release Inhibitor

Background

HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. HpARI is a new type of reagent to prevent IL-33-mediated pathology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.