Horse IgG, Fab2 Native Protein

SDS-Page: Horse IgG, Fab2 Native Protein [NBP1-96781] - SDS-PAGE of Horse F(ab')2 Fragment. Lane 1: 1x Reduced, Horse F(ab')2 Fragment. Lane 2: Molecular Weight Ladder. 4-20% SDS-PAGE; Coomassie Stained.

• Reactivity: Eq
• Applications: WB, PAGE

Horse IgG, Fab2 Native Protein Summary

• Description: Native Equine Horse IgG, Fab2
  
  Source: Normal serum
  
  Store vial at 4C upon receipt. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, anti-Horse IgG F(ab')2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot 1:100-1:2000
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.