| Dilutions |
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| Application Notes | Whole cell lysate provided for immunoprecipitation; for subsequent WB use, resuspend pellet in 80-800 ul electrophoresis sample buffer and load up to 20 ul per lane. If reducing conditions are desired, add a reducing agent (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)) prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RlPA buffer containing protease inhibitors |
| Concentration | 2.5 mg/ml |
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