HIV-1 Gag p24 Quantikine ELISA Kit

Product Discontinued

HIV-1 Gag p24 Quantikine ELISA Kit Summary

• Background: The Quantikine® QuicKit™ Human CD14 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human CD14 levels in cell culture supernates, serum, and plasma. It contains CHO-expressed recombinant human CD14 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human CD14 showed linear curves that were parallel to the standard cur...ves obtained using the recombinant QuicKit™ standards. These results indicate that this kit can be used determine relative mass values for natural CD14.
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• Specificity: Natural and recombinant human CD14.
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: gag

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIV-1 Gag p24 Quantikine ELISA Kit

• CA
• Capsid Protein
• HIV1 Gag p24
• HIV-1 Gag p24
• Pr55(Gag)

Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55^Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for HIV-1 Gag p24 (DHP240)(11)

Publications using DHP240

• L Vargas, N Valdivieso, F Tempio, V Simon, D Sauma, L Valenzuela, C Beltrán, L Castillo-D, X Contreras-, ML Acevedo, J Acevedo, RI Gonzalez, F Valiente-E, R Soto-Rifo, M Rosemblatt, M Lopez, F Osorio, MR Bono Serological study of CoronaVac vaccine and booster doses in Chile: immunogenicity and persistence of anti-SARS-CoV-2 spike antibodies Bmc Medicine, 2022-06-09;20(1):216. 2022-06-09 [PMID: 35676738] (Human)
• ML Acevedo, A Gaete-Arge, L Alonso-Pal, MM de Oca, A Bustamante, A Gaggero, F Paredes, CP Cortes, S Pantano, C Martínez-V, J Angulo, N Le Corre, M Ferrés, MA Navarrete, F Valiente-E, R Soto-Rifo Differential neutralizing antibody responses elicited by CoronaVac and BNT162b2 against SARS-CoV-2 Lambda in Chile Nature Microbiology, 2022-04-01;7(4):524-529. 2022-04-01 [PMID: 35365787] (Human)
• C Pereira-Mo, D Toro-Ascuy, C Ananías-Sá, A Gaete-Arge, C Rojas-Fuen, S Riquelme-B, B Rojas-Aray, F García-de-, P Aguilera-C, J Chnaiderma, ML Acevedo, F Valiente-E, R Soto-Rifo Epitranscriptomic regulation of HIV-1 full-length RNA packaging Nucleic Acids Research, 2022-02-28;0(0):. 2022-02-28 [PMID: 35137199] (Human)
• EA Ozer, LM Simons, OM Adewumi, AA Fowotade, EC Omoruyi, JA Adeniji, OA Olayinka, TJ Dean, J Zayas, PP Bhimalli, MK Ash, AI Maiga, AM Somboro, M Maiga, A Godzik, JR Schneider, JI Mamede, BO Taiwo, JF Hultquist, R Lorenzo-Re Multiple expansions of globally uncommon SARS-CoV-2 lineages in Nigeria Nature Communications, 2022-02-03;13(1):688. 2022-02-03 [PMID: 35115515] (Human)
• B Bhargavan, SM Woollard, JE McMillan, GD Kanmogne CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice Molecular Neurodegeneration, 2021-11-22;16(1):78. 2021-11-22 [PMID: 34809709] (Mouse)
• EA Ozer, LM Simons, OM Adewumi, AA Fowotade, EC Omoruyi, JA Adeniji, TJ Dean, J Zayas, PP Bhimalli, MK Ash, A Godzik, JR Schneider, JI Mamede, BO Taiwo, JF Hultquist, R Lorenzo-Re High prevalence of SARS-CoV-2 B.1.1.7 (UK variant) and the novel B.1.5.2.5 lineage in Oyo State, Nigeria medRxiv : the preprint server for health sciences, 2021-07-02;0(0):. 2021-07-02 [PMID: 33880483] (Human)
• O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype PloS Pathogens, 2021-04-19;17(4):e1009522. 2021-04-19 [PMID: 33872331] (Human)
• JI Mamede, J Griffin, S Gambut, TJ Hope A New Generation of Functional Tagged Proteins for HIV Fluorescence Imaging Viruses, 2021-02-28;13(3):. 2021-02-28 [PMID: 33670986] (Human)
• C Beltrán-Pa, S Riquelme-B, A Oyarzún-Ar, A Gaete-Arge, R González-S, K Cereceda-S, A Aguirre, D Travisany, R Palma-Veja, GP Barriga, A Gaggero, C Martínez-V, NL Corre, M Ferrés, ME Balcells, J Fernandez, E Ramírez, F Villarroel, F Valiente-E, R Soto-Rifo Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile Science Advances, 2021-02-12;7(7):. 2021-02-12 [PMID: 33579701] (Human)
• F Regent, L Morizur, L Lesueur, W Habeler, A Plancheron, K Ben M'Bare, C Monville Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions Sci Rep, 2019-07-23;9(1):10646. 2019-07-23 [PMID: 31337830] (Human)
• B Bhargavan, GD Kanmogne Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses Sci Rep, 2019-07-23;9(1):10689. 2019-07-23 [PMID: 31337802] (Human)

FAQs for HIV-1 Gag p24 (DHP240). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: gag