HIV-1 Gag p24 DuoSet ELISA, 5 Plate

• Reactivity: V
• Applications: ELISA
• Conjugate: Biotin

HIV-1 Gag p24 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIV-1 Gag p24 DuoSet ELISA, 5 Plate

• CA
• Capsid Protein
• HIV1 Gag p24
• HIV-1 Gag p24
• Pr55(Gag)

Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55^Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Publications for HIV-1 Gag p24 (DY7360-05)(6)

Publications using DY7360-05

• Chaphekar, D;Fernandes, C;Persaud, AT;Guzzo, C; Comparing methods to detect cellular proteins on the surface of HIV-1 virions Journal of virological methods 2024-12-06 [PMID: 39647666] (Human)
• Laomeephol, C;Tawinwung, S;Suppipat, K;Arunmanee, W;Wang, Q;Amie Luckanagul, J; Surface functionalization of virus-like particles via bioorthogonal click reactions for enhanced cell-specific targeting International journal of pharmaceutics 2024-06-10 [PMID: 38866085] (N/A)
• R Zhu, Q Meng, H Zhang, G Zhang, LSM Huang, Y Xu, RT Schooley, J An, Z Huang IV-1 gp120-CXCR4 recognition probed with synthetic nanomolar affinity D-peptides containing fragments of gp120 V3 loop European Journal of Medicinal Chemistry, 2022-09-28;244(0):114797. 2022-09-28 [PMID: 36270088] (Human)
• Y Chen, TB Lear, JW Evankovich, MB Larsen, B Lin, I Alfaras, JR Kennerdell, L Salminen, DP Camarco, KC Lockwood, F Tuncer, J Liu, MM Myerburg, JF McDyer, Y Liu, T Finkel, BB Chen A high-throughput screen for TMPRSS2 expression identifies FDA-approved compounds that can limit SARS-CoV-2 entry Nature Communications, 2021-06-23;12(1):3907. 2021-06-23 [PMID: 34162861] (Human)
• SH Tseng, B Lam, YJ Kung, J Lin, L Liu, YC Tsai, L Ferrall, RBS Roden, TC Wu, CF Hung A novel pseudovirus-based mouse model of SARS-CoV-2 infection to test COVID-19 interventions Journal of Biomedical Science, 2021-04-30;28(1):34. 2021-04-30 [PMID: 33926459] (Human)
• J Burnie, VA Tang, JA Welsh, AT Persaud, L Thaya, JC Jones, C Guzzo Flow Virometry Quantification of Host Proteins on the Surface of HIV-1 Pseudovirus Particles Viruses, 2020-11-12;12(11):. 2020-11-12 [PMID: 33198254] (Human Immunodeficiency Virus)

FAQs for HIV-1 Gag p24 (DY7360-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.