HIV-1 Gag p24 Antibody (749140) [Unconjugated]

Western blot shows recombinant HIV Gag p24 (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB7360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 749140
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HIV-1 Gag p24 Antibody (749140) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant viral HIV-1 Gag p24
  Pro149-Gly354
  Accession # NP_057850
• Specificity: Detects viral HIV-1 Gag p24 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: gag
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIV-1 Gag p24 Antibody (749140) [Unconjugated]

• CA
• Capsid Protein
• HIV1 Gag p24
• HIV-1 Gag p24
• Pr55(Gag)

Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIV-1 Gag p24 Antibody (MAB7360)(6)

Publications using MAB7360

• Bayraktar, C;Kayabolen, A;Odabas, A;Durgun, A;Kok, I;Sevinc, K;Supramaniam, A;Idris, A;Bagci-Onder, T; ACE2-Decorated Virus-Like Particles Effectively Block SARS-CoV-2 Infection International journal of nanomedicine 2024-07-10 [PMID: 39005960] (Western Blot, Human)
• Stacy Gellenoncourt, Nell Saunders, Rémy Robinot, Lucas Auguste, Maaran Michael Rajah, Jérôme Kervevan et al. The Spike-Stabilizing D614G Mutation Interacts with S1/S2 Cleavage Site Mutations To Promote the Infectious Potential of SARS-CoV-2 Variants Journal of Virology 2022-10-12 [PMID: 36121299]
• Toshiyuki Oda, Haruaki Yanagisawa, Hideyuki Shinmori, Youichi Ogawa, Tatsuyoshi Kawamura Cryo-electron tomography of Birbeck granules reveals the molecular mechanism of langerin lattice formation eLife 2022-06-27 [PMID: 35758632] (Western Blot, Human)
• B Meyer, J Chiaravall, S Gellenonco, P Brownridge, DP Bryne, LA Daly, A Grauslys, M Walter, F Agou, LA Chakrabart, CS Craik, CE Eyers, PA Eyers, Y Gambin, AR Jones, E Sierecki, E Verdin, M Vignuzzi, E Emmott Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential Nature Communications, 2021-09-21;12(1):5553. 2021-09-21 [PMID: 34548480] (Western Blot, Human)
• M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis PloS Pathogens, 2021-04-19;17(4):e1009526. 2021-04-19 [PMID: 33872329] (Western Blot, Human)
• Hydroxyproline-containing collagen peptide derived from the skin of the Alaska pollack inhibits HIV-1 infection Mol Med Rep, 2016-11-16;14(6):5489-5494. 2016-11-16 [PMID: 27878297] (Western Blot, Human)

Bioinformatics

• Gene Symbol: gag
• Uniprot:
  • NP_057850()