Recombinant HIV-1 Envelope Protein

Product Discontinued

Recombinant HIV-1 Envelope Protein Summary

• Description: Recombinant HIV-1 Envelope protein.

  Source: E. coli

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Application Notes: WB, ELISA This antigen is an E. coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and a the C-terminus of gp120. The gene encoding this fusion protein was synthesized using codons optimized for E.coli expression and does not represent a linear HIV-I envelope sequence. rHIV-I is a non-glycosylated, 233 aa polypeptide chain, having a molecular mass of 27275.88, pI=9.68.
• Theoretical MW: 27.27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.5X PBS & 6M urea
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Background

HIV-1 envelope is an E.coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and a small portion of gp120. The gene encoding this fusion protein was synthesized using codons optimized for E.coli expression and doesn't represent a linear HIV-1 envelope sequence. HIV-1 is a non-glycosylated, 233 amino acid polypeptide chain, having a molecular mass of 27275.88 dalton and pI=9.68. HIV-1 envelope protein spans the C-Terminus of gp120 and most of gp41. Superior diagnostic reagent for HIV-1 and HIV type-O detection. Detects all HIV-1 and HIV-type O infected individuals responding to envelope proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.