Histone

Product Discontinued

Histone Summary

• Immunogen: Histone, from calf thymus
• Preparation Method: Unfractionated whole histone

Applications/Dilutions

• Application Notes: This product is useful for Activation of Toll-like Receptor 2 (TLR2).timulation of TLR2 has been reported with 10-200 ug/ml. Note: TLR2 activation at this range of concentrations of Histone has also been observed in our product validation tests as shown in images

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Reconstituted in dH2O
• Preservative: No Preservative
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for Histone

• Histone (TLR2-Ligand)
• Histone TLR2 Ligand
• Histone
• Histone/TLR2 Ligand

Background

Histones are a group of alkaline proteins found in eukaryotic cell nuclei which form reversible complexes with DNA called nucleosomes. Studies show that extracellular histones are major mediators of death and sepsis.1 Furthermore, TLR2 and TLR4 have been determined as the major receptors for extracellular histone mediated sterile inflammation, tissue injury, and death in mouse model systems.2 Product Validation The following data show that Histone activated TLR2, of which EC50 was determined as 159.2 ug/ml by doseresponse assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.