Histone Ab ELISA Kit (Colorimetric) Summary
| Description |
Histone Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies to histone in human serum or plasma. |
| Standard Curve Range |
0-200 U/mL |
| Sensitivity |
1.0 U/mL |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 4.6-5.3% |
| Intra-Assay |
CV% < 3.8-4.6% |
| Sample Volume |
100 uL (prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- Calibrators A-F (0, 12.5, 25, 50, 100, 200 U/ml), containing serum/buffer matrix (PBS,BSA, detergent, NaN3 0.09%),yellow. Ready to use.
- Control positive and (1) negative (2), containing histone antibodies in a serum/buffermatrix (PBS, BSA, detergent, NaN3 0.09%, yellow. Ready to use.
- Divisible microplate: Consisting of 12 modules of 8 wells each. Ready to use.
- Enzyme Conjugate containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.
- Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, 5xconc.
- Stop solution (contains acid). Ready to use.
- TMB Substrate; containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
- Wash solution, containing Tris, detergent, preservative NaN3 0.09%; 50 x conc.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Histone Ab ELISA Kit (KA1120). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
