HistoGreen HRP Substrate

• Applications: Func, IHC

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Background

HistoGreen is a very sensitive substrate chromogen for use in peroxidase-based immunohistochemical (IHC) and in situ-hybridisation (ISH) staining procedures. It is perfectly suitable for single as well as for multi staining. The HistoGreen substrate kit contains all the reagents necessary to prepare the working solution for application. HistoGreen produces with the peroxidase a green reaction product that can be only permanently mounted. It produces with peroxidase and alkaline phosphatase substrates an excellent contrast and is compatible with the counterstains HistoHematoxylin and HistoNuclear Fast Red. In contrast to DAB, HistoGreen is neither toxic nor carcinogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for HistoGreen HRP Substrate (E109)(2)

Publications using E109

• Jonathan Gaucher, Emilie Montellier, Guillaume Vial, Florent Chuffart, Maëlle Guellerin, Sophie Bouyon, Emeline Lemarie, Yoshiki Yamaryo Botté, Aya Dirani, Raoua Ben Messaoud, Marie Joyeux Faure, Diane Godin Ribuot, Charlotte Costentin, Renaud Tamisier, Cyrille Y. Botté, Saadi Khochbin, Sophie Rousseaux, Jean-Louis Pépin Long-term intermittent hypoxia in mice induces inflammatory pathways implicated in sleep apnea and steatohepatitis in humans iScience 2024-01-09 [PMID: 38303705]
• Jonathan Gaucher, Guillaume Vial, Emilie Montellier, Maëlle Guellerin, Sophie Bouyon, Emeline Lemarie, Véronique Pelloux, Anne Bertrand, Karin Pernet-Gallay, Frederic Lamarche, Anne-Laure Borel, Claire Arnaud, Elise Belaidi, Karine Clément, Diane Godin Ribuot, Judith Aron-Wisnewsky, Jean-Louis Pépin Intermittent Hypoxia Rewires the Liver Transcriptome and Fires up Fatty Acids Usage for Mitochondrial Respiration Frontiers in Medicine 2022-02-18 [PMID: 35252260]