Histamine ELISA Kit (Colorimetric)

ELISA: Histamine ELISA Kit (Colorimetric) [NBP2-62860] - Samples were spiked with high concentrations of Histamine and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Histamine ELISA Kit (Colorimetric) [NBP2-62860] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Histamine ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes HIS in samples. No significant cross-reactivity or interference between HIS and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.58%
• Intra-Assay: CV% < 4.66%
• Spike Recovery: 87-105%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Histamine ELISA Kit (Colorimetric)

• 2 (4 imidazolyl)ethylamine
• 4 (2 aminoethyl) 1 3 diazole

Background

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Histamine ELISA Kit (NBP2-62860)(2)

Publications using NBP2-62860

• Yong P, Yip A, Islam F et al. The anti-allergic potential of stingless bee honey from different botanical sources via modulation of mast cell degranulation Research Square 2023-03-28 [PMID: 37667314] (ELISA)
• Tao R, Fu Z, Xiao L Chronic Food Antigen-specific IgG-mediated Hypersensitivity Reaction as A Risk Factor for Adolescent Depressive Disorder. Genomics Proteomics Bioinformatics. 2019-06-21 [PMID: 31233833] (ELISA, Human)

FAQs for Histamine ELISA Kit (NBP2-62860). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.