Histamine ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1888
    • Availability
      Product Discontinued

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Histamine ELISA Kit (Colorimetric) Summary

Description
Histamine ELISA Kit is an enzyme immunoassay for the quantitative determination of Histamine in stool.
Standard Curve Range
< 600 ng/g
Sensitivity
75 ng/g
Assay Type
Competitive ELISA
Inter-Assay
CV% < 6.8%
Intra-Assay
CV% < 9.3%
Spike Recovery
1.03
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Histamine ELISA Kit is an enzyme immunoassay for the quantitative determination of Histamine in stool.
Publications
Read Publication using KA1888.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Acylation Buffer: Ready to use, TRIS-buffer.
  2. Acylation Reagent: Ready to use, acylation reagent containing DMSO.
  3. Diluent: Ready to use, acidic buffer with non-mercury preservatives.
  4. Enzyme Conjugate: Ready to use, donkey anti-goat immunoglobulinsconjugated with peroxidase.
  5. Histamine Microtiter Strips: Ready to use, antigen precoated microwell plate in aresealable foil pouch with desiccant.
  6. Reaction Plate: Ready to use.
  7. Stop Solution: Ready to use, containing 0.25 M H2SO4.
  8. Substrate: Ready to use, chromogenic substrate containingtetramethylbenzidine, substrate buffer and hydrogen peroxide.
  9. Wash buffer Concentrate (50x): Concentrated buffer with a non-ionic detergentand physiological pH.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Histamine ELISA Kit (Colorimetric)

  • 2 (4 imidazolyl)ethylamine
  • 4 (2 aminoethyl) 1 3 diazole

Background

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Histamine ELISA Kit (KA1888)(1)

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