Histamine ELISA Kit (Colorimetric) Summary
Description |
Histamine ELISA Kit is an enzyme immunoassay for the quantitative determination of Histamine in stool. |
Standard Curve Range |
< 600 ng/g |
Sensitivity |
75 ng/g |
Assay Type |
Competitive ELISA |
Inter-Assay |
CV% < 6.8% |
Intra-Assay |
CV% < 9.3% |
Spike Recovery |
1.03 |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Dilutions |
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Application Notes |
Histamine ELISA Kit is an enzyme immunoassay for the quantitative determination of Histamine in stool. |
Publications |
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Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Acylation Buffer: Ready to use, TRIS-buffer.
- Acylation Reagent: Ready to use, acylation reagent containing DMSO.
- Diluent: Ready to use, acidic buffer with non-mercury preservatives.
- Enzyme Conjugate: Ready to use, donkey anti-goat immunoglobulinsconjugated with peroxidase.
- Histamine Microtiter Strips: Ready to use, antigen precoated microwell plate in aresealable foil pouch with desiccant.
- Reaction Plate: Ready to use.
- Stop Solution: Ready to use, containing 0.25 M H2SO4.
- Substrate: Ready to use, chromogenic substrate containingtetramethylbenzidine, substrate buffer and hydrogen peroxide.
- Wash buffer Concentrate (50x): Concentrated buffer with a non-ionic detergentand physiological pH.
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Histamine ELISA Kit (Colorimetric)
Background
Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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