His Tag Antibody (AD1.1.10) [Allophycocyanin]

(A) Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (Catalog # IC050A). (B) No staining was observed in the absence of protein. Staining was performed using our Staining Membrane-Associated Proteins protocol.

HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: All-Multi
• Applications: Flow, Flow
• Clone: AD1.1.10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

His Tag Antibody (AD1.1.10) [Allophycocyanin] Summary

• Immunogen: His-tagged peptide
• Specificity: Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for His Tag Antibody (AD1.1.10) [Allophycocyanin]

• 6 His epitope tag
• 6-His Tag
• 6X His Tag
• 6X His
• 6x-His Tag
• 6X-His
• H
• Hexa His tag
• HHHHHH epitope tag
• HHHHHH tag
• His Tag
• HIS
• His6-Tag
• polyhistidine tag
• polyHistidine
• Poly-histidine

Background

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (IC050A)(6)

Publications using IC050A

• Vivek Subbiah, Sant P. Chawla, Anthony P. Conley, Breelyn A. Wilky, Anthony Tolcher, Nehal J. Lakhani et al. Preclinical Characterization and Phase I Trial Results of INBRX-109, A Third-Generation, Recombinant, Humanized, Death Receptor 5 Agonist Antibody, in Chondrosarcoma Clinical Cancer Research 2023-08-15 [PMID: 37265425]
• WN Liu, WY So, SL Harden, SY Fong, MXY Wong, WWS Tan, SY Tan, JKL Ong, R Rajarethin, M Liu, JY Cheng, L Suteja, JPS Yeong, NG Iyer, DW Lim, Q Chen Successful targeting of PD-1/PD-L1 with chimeric antigen receptor-natural killer cells and nivolumab in a humanized mouse cancer model Science Advances, 2022-11-23;8(47):eadd1187. 2022-11-23 [PMID: 36417514] (Flow Cytometry, Xenograft)
• Michael Klichinsky, Marco Ruella, Olga Shestova, Xueqing Maggie Lu, Andrew Best, Martha Zeeman et al. Human chimeric antigen receptor macrophages for cancer immunotherapy Nature Biotechnology 2020-08-01 [PMID: 32361713]
• A Cho, AL Caldara, NA Ran, Z Menne, RC Kauffman, M Affer, A Llovet, C Norwood, A Scanlan, G Mantus, B Bradley, S Zimmer, T Schmidt, M Hertl, AS Payne, R Feldman, AP Kowalczyk, J Wrammert Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease Cell Rep, 2019-07-23;28(4):909-922.e6. 2019-07-23 [PMID: 31340153] (Flow Cytometry, Human)
• Katherine Griffiths, Uli Binder, William McDowell, Rita Tommasi, Mark Frigerio, William G. Darby et al. Half-life extension and non-human primate pharmacokinetic safety studies of i-body AD-114 targeting human CXCR4 mAbs 2019-10-03 [PMID: 31156041] (Flow Cytometry, Human)
• T Osada, ZC Hartman, J Wei, G Lei, AC Hobeika, WR Gwin, MA Diniz, N Spector, TM Clay, W Chen, MA Morse, HK Lyerly Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers Breast Cancer Res., 2018-08-09;20(1):90. 2018-08-09 [PMID: 30092835] (Binding Assay, Human)