His Tag Antibody (3D5) [Alexa Fluor® 750]


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Product Details

Reactivity All-NASpecies Glossary
Applications WB, ELISA
Armenian Hamster
Alexa Fluor 750

His Tag Antibody (3D5) [Alexa Fluor® 750] Summary

This His Tag Antibody (3D5) - Chimeric was developed against a purified his-tagged Fab fragment.
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
IgG Kappa
Armenian Hamster
Protein A purified
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  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for His Tag Antibody (3D5) [Alexa Fluor® 750]

  • 6 His epitope tag
  • 6-His Tag
  • 6X His Tag
  • 6X His
  • 6x-His Tag
  • 6X-His
  • H
  • Hexa His tag
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • His6-Tag
  • polyhistidine tag
  • polyHistidine
  • Poly-histidine


A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).


1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-81022AF350
Alexa Fluor 405 NBP2-81022AF405
Alexa Fluor 488 NBP2-81022AF488
Alexa Fluor 532 NBP2-81022AF532
Alexa Fluor 594 NBP2-81022AF594
Alexa Fluor 647 NBP2-81022AF647
Alexa Fluor 700 NBP2-81022AF700
Alexa Fluor 750 NBP2-81022AF750
Biotin NBP2-81022B
DyLight 350 NBP2-81022UV
DyLight 405 NBP2-81022V
DyLight 488 NBP2-81022G
DyLight 550 NBP2-81022R
DyLight 594 NBP2-81022DL594
DyLight 650 NBP2-81022C
DyLight 680 NBP2-81022FR
DyLight 755 NBP2-81022IR
FITC NBP2-81022F
HRP NBP2-81022H

Additional His Tag Products

Array NBP2-81022AF750

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