Human Herpes Simplex Virus II Antibody (IgM) ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of human herpes simplexvirus II IgM antibody (HSVII-Ab) No significant cross-reactivity or interference between human herpes simplexvirus II IgM antibody (HSVII -Ab) and analogues was observed. |
Standard Curve Range |
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Assay Type |
Indirect ELISA |
Inter-Assay |
CV% < 15% |
Intra-Assay |
CV% < 15% |
Sample Volume |
50-100ul |
Kit Type |
ELISA Kit (Colorimetric) |
Packaging, Storage & Formulations
Kit Components
Components
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- Substrate B
- Substrate A
- Sample Diluent
- Stop Solution
- Wash Buffer (20 x concentrate)
- Coated assay plate
- Adhesive Strip (For 96 wells)
- Positive Control
- Negative Control
- Instruction manual
- HRP-conjugate
|
Notes
Please contact our Technical Services with any questions regarding species reactivity
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human herpes simplexvirus II IgM antibody (HSVII-Ab) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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