Hepatocyte Specific Antigen Antibody (EP-1)

Product Discontinued

Hepatocyte Specific Antigen Antibody (EP-1) Summary

• Immunogen: The antibody reacts with the Hepatocytes antigen.
• Specificity: Monoclonal antibody EP-1 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind EP-1 on the cell surface. Monoclonal antibody EP-1 stains liver canaliculi in normal liver sections and is positive on the cell surface of human liver carcinomas. Monoclonal antibodies to liver cell processes are useful in the identification of hepatic carcinomas and normal organ structures. Two monoclonal antibodies to different liver substructures are offered which bind to subsets of hepatic carcinomas.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-10-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: For use with paraformaldehyde/acetone fixed cell preparations or frozen tissues. Liver may be used as a positive control tissue. Monoclonal antibody EP-1 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin sections.

Reactivity Notes

Human,Rat

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1 mg/ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Hepatocyte Specific Antigen Antibody (EP-1)

• Hepatocyte
• HSA

Background

HSA (Human Serum Albumin) is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. It labels an antigen in the mitochondrial fraction of the liver homogenates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.