Human Hepatitis D Virus Antigen ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of human hepatitis D virus(HDV) antigen. No significant cross-reactivity or interference between human hepatitis D virus(HDV) antigen and analogues was observed. |
Standard Curve Range |
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Sensitivity |
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Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 15 |
Intra-Assay |
%CV < 15 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
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- Adhesive Strip (For 96 wells)
- Antigen Lysate Buffer
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (30 x concentrate)
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Notes
Please contact our Technical Services with any questions regarding species reactivity
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase (HRP). Any HDV antigen present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human hepatitis D virus(HDV) antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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