Human Hepatitis B Virus Core Antibody ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of human anti-hepatitis B virus core antibody(HBcAb) No significant cross-reactivity or interference between human anti-hepatitis B virus core antibody(HBcAb) and analogues was observed. |
Assay Type |
Competitive ELISA |
Inter-Assay |
%CV < 20 |
Intra-Assay |
%CV < 20 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
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- Adhesive Strip (For 96 wells)
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (20 x concentrate)
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Alternate Names for Human Hepatitis B Virus Core Antibody ELISA Kit (Colorimetric)
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBcAg. Samples are pipetted into the wells with HBcAb conjugated Horseradish Peroxidase (HRP). Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of human anti-hepatitis B virus core antibody(HBcAb) in the samples. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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