Human Hepatitis A Virus Antibody (IgM) ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum
Standard Curve Range
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Order Details

Human Hepatitis A Virus Antibody (IgM) ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human anti-HAV antibody (IgM). No significant cross-reactivity or interference between human anti-HAV antibody (IgM) and analogues was observed.
Standard Curve Range
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Assay Type
capture ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 15%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Substrate A
  2. Substrate B
  3. Stop Solution
  4. Sample Diluent
  5. Wash Buffer (20 x concentrate)
  6. Coated assay plate
  7. Adhesive Strip
  8. Negative Control
  9. Positive Control
  10. HRP-conjugate
  11. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-human IgM. Samples are pipetted into the wells and then human anti-HAV antibody (IgM) in sample is captured. After wash, add HAV antigen conjugated Horseradish Peroxidase (HRP) into the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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