Human Hepatitis A Virus Antibody (IgM) ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of human anti-HAV antibody (IgM). No significant cross-reactivity or interference between human anti-HAV antibody (IgM) and analogues was observed. |
Standard Curve Range |
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Assay Type |
capture ELISA |
Inter-Assay |
CV% < 15% |
Intra-Assay |
CV% < 15% |
Sample Volume |
50-100ul |
Kit Type |
ELISA Kit (Colorimetric) |
Packaging, Storage & Formulations
Kit Components
Components
|
- Substrate A
- Substrate B
- Stop Solution
- Sample Diluent
- Wash Buffer (20 x concentrate)
- Coated assay plate
- Adhesive Strip
- Negative Control
- Positive Control
- HRP-conjugate
- Instruction manual
|
Notes
Please contact our Technical Services with any questions regarding species reactivity
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-human IgM. Samples are pipetted into the wells and then human anti-HAV antibody (IgM) in sample is captured. After wash, add HAV antigen conjugated Horseradish Peroxidase (HRP) into the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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