Human Hepatitis A Virus Antibody (IgG) ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum
Standard Curve Range
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Order Details

Human Hepatitis A Virus Antibody (IgG) ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human anti-HAV antibody (IgG). No significant cross-reactivity or interference between human anti-HAV antibody (IgG) and analogues was observed.
Standard Curve Range
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Assay Type
Indirect ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 15%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. HRP-conjugate
  2. Instruction manual
  3. Positive Control
  4. Negative Control
  5. Adhesive Strip
  6. Coated assay plate
  7. Wash Buffer (20 x concentrate)
  8. Sample Diluent
  9. Stop Solution
  10. Substrate B
  11. Substrate A

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HAV antigen. Samples are pipetted into the wells with mouse anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibody specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-HAV antibody (IgG) bound in the intial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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