Hepatitis A Surface Antigen Antibody (BGN/B5B3 (B5-B3)) [CoraFluor™ 1]

• Reactivity: V-Vi
• Applications: ELISA, Func
• Clone: BGN/B5B3 (B5-B3)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Hepatitis A Surface Antigen Antibody (BGN/B5B3 (B5-B3)) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Lysate from foetal kidney cells of rhesus monkey infected with extract from patient with active viral Hepatitis A
• Specificity: NB100-62197 is specific for Hepatitis A surface antigen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Radioimmunoassay
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Viral. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Background

Hepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form three (possibly four) capsid proteins and several nonstructural proteins. HAV genomic replication occurs exclusively in the cytoplasm of the infected hepatocyte by a mechanism involving an RNA-dependent RNA polymerase. Though he clinical picture of viral (A) hepatitis is extremely variable - ranging from asymptotic infection without jaundice to a fulminating disease and death in a few days, Hepatitis A is usually a mild illness and does not lead to chronic or persistent hepatitis. HAV is transmitted by the orofecal route and is still endemic throughout much of the world, including the western world. HAV exists worldwide as a single serotype with a small degree of antigenetic variation. Present evidence suggests that immunity acquired naturally (or from an inactivated hepatitis A vaccine) will protect against all human HAV strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.