Hep2 Whole Cell Lysate


Western Blot: Hep2 Whole Cell Lysate [NB800-PC7] - Coommassie stained SDS-PAGE of 20 ul of Human Derived Hep2 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB
1 mg/ml

Order Details

Hep2 Whole Cell Lysate Summary

Hep2 whole cell extract lysate

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
1 mg/ml


  • Western Blot
Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Lysate Details for Hep2

Subcellular Fraction


This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional Hep2 Whole Cell Lysate Products

Hep2 NB800-PC7

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