Hep2 Whole Cell Lysate

Images

 
Western Blot: Hep2 Whole Cell Lysate [NB800-PC7] - Coommassie stained SDS-PAGE of 20 ul of Human Derived Hep2 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
1 mg/ml

Order Details

Hep2 Whole Cell Lysate Summary

Specificity
Hep2 whole cell extract lysate

Applications/Dilutions

Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
Concentration
1 mg/ml

Lysate Details for Hep2

Type
Cell
Tissue
Reproductive
Subcellular Fraction
Whole

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Hep2 Lysate (NB800-PC7) (0)

There are no publications for Hep2 Lysate (NB800-PC7).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Hep2 Lysate (NB800-PC7) (0)

There are no reviews for Hep2 Lysate (NB800-PC7). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Hep2 Lysate (NB800-PC7) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Hep2 Products

Hep2 NB800-PC7

Blogs on Hep2

There are no specific blogs for Hep2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Hep2 Whole Cell Lysate and receive a gift card or discount.