HeLa S3 Nuclear Cell Lysate

Product Discontinued

HeLa S3 Nuclear Cell Lysate Summary

• Description: Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue. Nuclear extract cell lysate (non-denatured)
• Localization: Cervix; adenocarcinoma
• Preparation Method: Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2 mg/ml.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue
• Concentration: 2.5 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Reproductive
• Protein State: Native
• Subcellular Fraction: Nuclear

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.