Mouse HBsAg Antibody ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma

Order Details

Mouse HBsAg Antibody ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse hepatitis B virus surface antibody (HBsAb). No significant cross-reactivity or interference between mouse hepatitis B virus surface antibody (HBsAb) and analogues was observed.
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 10
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Coated assay plate
  3. HRP-conjugate
  4. Instruction manual
  5. Negative Control
  6. Positive Control
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20 x concentrate)

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBsAg. Samples are pipetted into the wells with HBsAg conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mouse hepatitis B virus surface antibody (HBsAb) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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FAQs for HBsAg Antibody ELISA Kit (NBP2-60036). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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